Document Details
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Document Type |
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Thesis |
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Document Title |
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Cytological and Biochemical Effects of Some Activating Products on Vicia faba Plant التأثيرات السيتولوجية والبيوكيميائية لبعض المنتجات المنشطة على نبات الفول |
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Subject |
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Cytological and Biochemical Effects of Some Activating Products on Vicia faba Plant |
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Document Language |
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Arabic |
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Abstract |
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Cytogenotoxicity effects for the three activating products were studied on Vicia faba plant with four concentrations,i.e: energy drink "bison"(50,80,90and100%);activator sport "creatine monohydrate" (1.5,2,2.5and 3g /100ml)and ovulation inducer drug "clomid" (25,50,100and 150mg/100ml)on Vicia faba plant (Giza 40) for three periods (6,12 and 24) hours for study the mitotic divisions behavior and estimate nucleic acid contents in the root tips,and also after (24,48) hours and 15 days from spraying Vicia faba plants (on the flowering stage) to study meiotic division behavior and determined leaf protein after 15 days from spraying with protein SDS-electrophoresis technique. On the other hand ,RAPD-PCR reaction was performed on( M2) treated Vicia faba plants with the highest concentration from each activating product. The most treatments caused significant reduction in mitotic index, unbalanced mitotic stages percentages, highly significant increasing of total mitotic abnormalities% and the greatest portion of this trait was formed by stickiness,whereas:total abnormalities%(10.9%-90.14%)with stickiness formed in it (4.00%-81.72%) in bison treatments, total abnormalities%(5.72%-66.46%)with stickiness formed in it (1.21%-55.20%) in creatine treatments while total abnormalities%(22.78%-83.52%)with stickiness formed in it (15.63%-62.63%) in clomeid treatments ,However, the residual portion percentage of this trait were formed by:C-metaphase, disturbed and laggards consequently. In addition, another mitotic abnormalities such as:multipolor, bridges, star ship, breaks and micronuclei were observed in some treatments but with low percentages. On the other hand, both mitotic index and total abnormalities % were affected by the concentration of material studied and exposure times in the most treatments. ALL different treatments revealed highly significant increasing of total meiotic abnormalities% which it affected by the concentration and the exposure time. Reduction of total meiotic abnormalities% were recorded in some treatments after 15 days from spraying comparing with (24 and 48)hours as a result of the recovery in this stage. Second meiotic abnormalities % was reduced than the first meiotic abnormalities% in some treatments as a result of the recovery in this stage. The greatest portion from total meiotic abnormalities were observed in metaphase and anaphase in both frist and second meiotic division. The dominant meiotic abnormalities were: stickiness, disturbed which showed in all meiotic stages, in addition, laggards, bridges, micronuclei were observed in some treatments but with low percentages. The most treatments alternated DNA and RNA contents, but the prolonged of exposure time reduced these traits in the most treatments. SDS-electrophoresis profile of leaf proteins showed changes in some intensity bands in all (bison or clomid) treatments. While, all creatine treatments caused disappearance of two bands(60 and 90KDa). RAPD-PCR reaction revealed alteration on DNA bands, whereas 150mg/100ml clomid alternated 21 bands (17 bands disappeared and 4 new bands appeared), while 100% bison changed 10 DNA bands (8bands disappeared and 2 new bands appeared), However, 3g /100 creatine affected of 9 DNA bands (5 bands disappeared and 4 new bands appeared). From, abovious results, we concluded that the three activating products have a cytogenotoxic effects and they arranged according to the highest toxicity as follows:clomid>bison>creatine monohydrate. Therefore, we should be avoid taking these activiting products. |
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Supervisor |
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Dr. Asmahan Ahmed Mahmoud Ali |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1432 AH
2011 AD |
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Number Of Pages |
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180 |
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Added Date |
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Wednesday, July 6, 2011 |
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Researchers
| صالحة مسفر الشمراني | AL-Shamrani, Salha Mesfer | Investigator | Doctorate | Sal-2006@hotmail.com |
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